shrnas against sox2 Search Results


anti human sox2 shrna gene set  (Genecopoeia)
94
Genecopoeia anti human sox2 shrna gene set
A ) Immunohistochemical staining of <t>Sox2</t> demonstrating representative nuclear basal epithelial staining (dark red) in normal glands (Region #3), and two distinct tumor regions that are either uniformly Sox2-positve (Region #2) or Sox2-negative (Region #1). B ) Expression of Sox2 in representative castration-resistant metastatic lesions. The box in the 8× magnification corresponds to the region shown in the 40× image. C ) Percentage and distribution of Sox2 expression among prostate disease states. In normal, BPH, and HGPIN tissues, Sox2 is expressed in basal-epithelial cells (Grey Bars). Positive expression is defined by more than 50% of the cancer cells positive with a relative intensity of 1 or more on a scale of 0–3. In cancer tissues and metastases, Sox2 is either uniformly expressed or absent, and the percentage of Sox2-positive tumors increases with Gleason Score (Black Bars). BPH: Benign Prostatic Hyperplasia; HGPIN: high-grade prostatic intraepithelial neoplasia; GS: Gleason Score (N = number of individual patient specimens analyzed).
Anti Human Sox2 Shrna Gene Set, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti human sox2 shrna gene set/product/Genecopoeia
Average 94 stars, based on 1 article reviews
anti human sox2 shrna gene set - by Bioz Stars, 2026-03
94/100 stars
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sox2-overexpressed lentivirus lenti-sox2  (Genechem)
90
Genechem sox2-overexpressed lentivirus lenti-sox2
A ) Immunohistochemical staining of <t>Sox2</t> demonstrating representative nuclear basal epithelial staining (dark red) in normal glands (Region #3), and two distinct tumor regions that are either uniformly Sox2-positve (Region #2) or Sox2-negative (Region #1). B ) Expression of Sox2 in representative castration-resistant metastatic lesions. The box in the 8× magnification corresponds to the region shown in the 40× image. C ) Percentage and distribution of Sox2 expression among prostate disease states. In normal, BPH, and HGPIN tissues, Sox2 is expressed in basal-epithelial cells (Grey Bars). Positive expression is defined by more than 50% of the cancer cells positive with a relative intensity of 1 or more on a scale of 0–3. In cancer tissues and metastases, Sox2 is either uniformly expressed or absent, and the percentage of Sox2-positive tumors increases with Gleason Score (Black Bars). BPH: Benign Prostatic Hyperplasia; HGPIN: high-grade prostatic intraepithelial neoplasia; GS: Gleason Score (N = number of individual patient specimens analyzed).
Sox2 Overexpressed Lentivirus Lenti Sox2, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sox2-overexpressed lentivirus lenti-sox2/product/Genechem
Average 90 stars, based on 1 article reviews
sox2-overexpressed lentivirus lenti-sox2 - by Bioz Stars, 2026-03
90/100 stars
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shrna clone set against human sox2  (Genecopoeia)
94
Genecopoeia shrna clone set against human sox2
A ) Immunohistochemical staining of <t>Sox2</t> demonstrating representative nuclear basal epithelial staining (dark red) in normal glands (Region #3), and two distinct tumor regions that are either uniformly Sox2-positve (Region #2) or Sox2-negative (Region #1). B ) Expression of Sox2 in representative castration-resistant metastatic lesions. The box in the 8× magnification corresponds to the region shown in the 40× image. C ) Percentage and distribution of Sox2 expression among prostate disease states. In normal, BPH, and HGPIN tissues, Sox2 is expressed in basal-epithelial cells (Grey Bars). Positive expression is defined by more than 50% of the cancer cells positive with a relative intensity of 1 or more on a scale of 0–3. In cancer tissues and metastases, Sox2 is either uniformly expressed or absent, and the percentage of Sox2-positive tumors increases with Gleason Score (Black Bars). BPH: Benign Prostatic Hyperplasia; HGPIN: high-grade prostatic intraepithelial neoplasia; GS: Gleason Score (N = number of individual patient specimens analyzed).
Shrna Clone Set Against Human Sox2, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrna clone set against human sox2/product/Genecopoeia
Average 94 stars, based on 1 article reviews
shrna clone set against human sox2 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


A ) Immunohistochemical staining of Sox2 demonstrating representative nuclear basal epithelial staining (dark red) in normal glands (Region #3), and two distinct tumor regions that are either uniformly Sox2-positve (Region #2) or Sox2-negative (Region #1). B ) Expression of Sox2 in representative castration-resistant metastatic lesions. The box in the 8× magnification corresponds to the region shown in the 40× image. C ) Percentage and distribution of Sox2 expression among prostate disease states. In normal, BPH, and HGPIN tissues, Sox2 is expressed in basal-epithelial cells (Grey Bars). Positive expression is defined by more than 50% of the cancer cells positive with a relative intensity of 1 or more on a scale of 0–3. In cancer tissues and metastases, Sox2 is either uniformly expressed or absent, and the percentage of Sox2-positive tumors increases with Gleason Score (Black Bars). BPH: Benign Prostatic Hyperplasia; HGPIN: high-grade prostatic intraepithelial neoplasia; GS: Gleason Score (N = number of individual patient specimens analyzed).

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) Immunohistochemical staining of Sox2 demonstrating representative nuclear basal epithelial staining (dark red) in normal glands (Region #3), and two distinct tumor regions that are either uniformly Sox2-positve (Region #2) or Sox2-negative (Region #1). B ) Expression of Sox2 in representative castration-resistant metastatic lesions. The box in the 8× magnification corresponds to the region shown in the 40× image. C ) Percentage and distribution of Sox2 expression among prostate disease states. In normal, BPH, and HGPIN tissues, Sox2 is expressed in basal-epithelial cells (Grey Bars). Positive expression is defined by more than 50% of the cancer cells positive with a relative intensity of 1 or more on a scale of 0–3. In cancer tissues and metastases, Sox2 is either uniformly expressed or absent, and the percentage of Sox2-positive tumors increases with Gleason Score (Black Bars). BPH: Benign Prostatic Hyperplasia; HGPIN: high-grade prostatic intraepithelial neoplasia; GS: Gleason Score (N = number of individual patient specimens analyzed).

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Immunohistochemical staining, Staining, Expressing

A ) Immunofluorescent co-staining of Sox2 (green) with the basal-specific marker p63 (red), showing that 75% of normal basal-epithelial cells are positive for both Sox2 and p63 (yellow) while the remaining 25% are Sox2-negative (red). DAPI staining highlights nuclei (representative images of normal prostate epithelium acquired from three different individual patient specimens). B ) Western blotting of a series of patient-derived Prostate (PrEC) and Seminal Vesicle (SVEC) epithelial sell (PrEC) cultures demonstrates that a portion of these cultures express detectable Sox2, while other PrEC and all SVEC cultures do not. C ) Immunocytochemical staining of Sox2 showing uniform nuclear Sox2 expression in Sox2-positive PrECs and lack of Sox2-positive cells within Sox2-negative PrECs (representative images of three independent experiments).

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) Immunofluorescent co-staining of Sox2 (green) with the basal-specific marker p63 (red), showing that 75% of normal basal-epithelial cells are positive for both Sox2 and p63 (yellow) while the remaining 25% are Sox2-negative (red). DAPI staining highlights nuclei (representative images of normal prostate epithelium acquired from three different individual patient specimens). B ) Western blotting of a series of patient-derived Prostate (PrEC) and Seminal Vesicle (SVEC) epithelial sell (PrEC) cultures demonstrates that a portion of these cultures express detectable Sox2, while other PrEC and all SVEC cultures do not. C ) Immunocytochemical staining of Sox2 showing uniform nuclear Sox2 expression in Sox2-positive PrECs and lack of Sox2-positive cells within Sox2-negative PrECs (representative images of three independent experiments).

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Staining, Marker, Western Blot, Derivative Assay, Expressing

A ) To test whether AR signaling impacted Sox2 expression, we ectopically expressed AR (LV-AR) and control lentivirus (LV-Control) in two different Sox2-positive PrEC lines (#1 and #2). Western blotting shows that expression and further ligand activation of AR using 1 nM R1881 results in decreased Sox2 protein expression and decreased p63 expression. GAPDH was used as a loading control. B ) To test whether AR-mediated inhibition of Sox2 expression was specific to PrECs or could also occur in Sox2-positive human ES cells, we treated the WA01(H1) human ES cell line with androgen (1 nM R1881). Western blotting documents detectable endogenous AR expression in hESCs, which increases in response to androgen. β-Actin was used as a loading control. C ) AR activation in hESCs results in a significant decrease in Sox2 mRNA expression as measured by qPCR (* indicates p<0.05). This was compared to untreated and vehicle treated cells (EtOH).

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) To test whether AR signaling impacted Sox2 expression, we ectopically expressed AR (LV-AR) and control lentivirus (LV-Control) in two different Sox2-positive PrEC lines (#1 and #2). Western blotting shows that expression and further ligand activation of AR using 1 nM R1881 results in decreased Sox2 protein expression and decreased p63 expression. GAPDH was used as a loading control. B ) To test whether AR-mediated inhibition of Sox2 expression was specific to PrECs or could also occur in Sox2-positive human ES cells, we treated the WA01(H1) human ES cell line with androgen (1 nM R1881). Western blotting documents detectable endogenous AR expression in hESCs, which increases in response to androgen. β-Actin was used as a loading control. C ) AR activation in hESCs results in a significant decrease in Sox2 mRNA expression as measured by qPCR (* indicates p<0.05). This was compared to untreated and vehicle treated cells (EtOH).

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Expressing, Control, Western Blot, Activation Assay, Inhibition

A ) Western blot of a panel of non-malignant and prostate cancer cell lines for Sox2, Nanog, Oct4, and AR. β-Actin was used as a loading control. Expression of Sox2 in castration-resistant CWR cells is not accompanied by co-expression of Nanog or Oct4. LNCaP, C4-2B, LAPC-4, and MDA-PCa2B cells expressed detectable Nanog, which is presumably the NanogP8 retrogene (Jeter et al., 2011). The human embryonal carcinoma cell line NCCIT was used as a positive control for Sox2, Nanog, and Oct4 at a 1∶10 dilution. B ) Decreased expression of Sox2 upon AR stimulation with physiologic levels of androgen (1 nM R1881) in castration-resistant CWR-R1 prostate cancer cells. Protein lysates from cells treated at defined intervals (3–48 hours) after androgen treatment were subjected to western blotting, and accumulation of secreted PSA expressed in the media validates increased AR signaling. C ) Rapid decrease of Sox2 mRNA in CWR-R1 cells upon AR stimulation as measured by qPCR. Levels at 0.5 hrs and beyond represent a statistically significant decrease in Sox2 mRNA (p<0.05). D ) AR Chromatin Immunoprecipitation (ChIP) documents direct binding of ligand-activated AR to the Sox2 enhancer region in response to AR stimulation by R1881. CWR-R1 cells were treated with vehicle control or 1 nM R1881, and enrichment of the Sox2 promoter after AR-ChIP was normalized as a percentage of total chromatin input. IgG and Histone H3 served as negative and positive controls, respectively. When compared to total input, both the positive control Histone H3 and ligand-activated AR significantly enriched for the Sox2 enhancer (p<0.05). Data represents three independent experiments.

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) Western blot of a panel of non-malignant and prostate cancer cell lines for Sox2, Nanog, Oct4, and AR. β-Actin was used as a loading control. Expression of Sox2 in castration-resistant CWR cells is not accompanied by co-expression of Nanog or Oct4. LNCaP, C4-2B, LAPC-4, and MDA-PCa2B cells expressed detectable Nanog, which is presumably the NanogP8 retrogene (Jeter et al., 2011). The human embryonal carcinoma cell line NCCIT was used as a positive control for Sox2, Nanog, and Oct4 at a 1∶10 dilution. B ) Decreased expression of Sox2 upon AR stimulation with physiologic levels of androgen (1 nM R1881) in castration-resistant CWR-R1 prostate cancer cells. Protein lysates from cells treated at defined intervals (3–48 hours) after androgen treatment were subjected to western blotting, and accumulation of secreted PSA expressed in the media validates increased AR signaling. C ) Rapid decrease of Sox2 mRNA in CWR-R1 cells upon AR stimulation as measured by qPCR. Levels at 0.5 hrs and beyond represent a statistically significant decrease in Sox2 mRNA (p<0.05). D ) AR Chromatin Immunoprecipitation (ChIP) documents direct binding of ligand-activated AR to the Sox2 enhancer region in response to AR stimulation by R1881. CWR-R1 cells were treated with vehicle control or 1 nM R1881, and enrichment of the Sox2 promoter after AR-ChIP was normalized as a percentage of total chromatin input. IgG and Histone H3 served as negative and positive controls, respectively. When compared to total input, both the positive control Histone H3 and ligand-activated AR significantly enriched for the Sox2 enhancer (p<0.05). Data represents three independent experiments.

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Western Blot, Control, Expressing, Positive Control, Chromatin Immunoprecipitation, Binding Assay

Levels of Sox2 mRNA.

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: Levels of Sox2 mRNA.

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques:

A ) To verify that the decrease of Sox2 protein is specific to AR activation and can be reversed by an AR antagonist, CWR-R1 cells were grown in 1 nm R1881 or vehicle for 24 hrs, and then either 10 µM MDV3100 or vehicle was added to the culture medium for an additional 48 hrs. Western blots show a decrease in Sox2 protein with R1881 that can be returned to basal levels with addition of MDV3100, without any change in AR protein. β-Actin was used as a loading control. A schematic outlines the time frame of these experiments. B ) A decrease of Sox2 mRNA with R1881 treatment was measured using qPCR (*p<0.05), which was brought back to control levels upon treatment with MDV3100. C ) A time course of treatment with a known inhibitor of transcription, 10 µM Actinomycin D, yielded a similar rapid decrease of Sox2 mRNA in CWR-R1 cells upon AR stimulation with R1881 as measured by qPCR. Levels at 0.25 hrs and beyond represent a statistically significant decrease in Sox2 mRNA (p<0.05), showing similar kinetics of transcriptional repression with both R1881 and Actinomycin D drug treatments.

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) To verify that the decrease of Sox2 protein is specific to AR activation and can be reversed by an AR antagonist, CWR-R1 cells were grown in 1 nm R1881 or vehicle for 24 hrs, and then either 10 µM MDV3100 or vehicle was added to the culture medium for an additional 48 hrs. Western blots show a decrease in Sox2 protein with R1881 that can be returned to basal levels with addition of MDV3100, without any change in AR protein. β-Actin was used as a loading control. A schematic outlines the time frame of these experiments. B ) A decrease of Sox2 mRNA with R1881 treatment was measured using qPCR (*p<0.05), which was brought back to control levels upon treatment with MDV3100. C ) A time course of treatment with a known inhibitor of transcription, 10 µM Actinomycin D, yielded a similar rapid decrease of Sox2 mRNA in CWR-R1 cells upon AR stimulation with R1881 as measured by qPCR. Levels at 0.25 hrs and beyond represent a statistically significant decrease in Sox2 mRNA (p<0.05), showing similar kinetics of transcriptional repression with both R1881 and Actinomycin D drug treatments.

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Activation Assay, Western Blot, Control

A ) To test whether Sox2 expression is associated with resistance to AR pathway inhibition, we developed a series of prostate cancer cell lines that were resistant to MDV3100. After 30 days of continuous treatment, MDV3100-resistant lines expressed significantly higher Sox2 as measured using qPCR (*p<0.05). AR-mediated PSA expression was also significantly reduced in these lines. B ) Comparison between the castration-sensitive LNCaP and isogenic castration-resistant C4-2 cell lines documents a significant increase in Sox2 expression in castration-resistant C4-2 cells (*p<0.05). C ) Increased Sox2 expression in castration-resistant xenografts of LAPC-4 and CWR-R1 tumors. Tumors were allowed to establish and then host mice were castrated; 30 days later castration-resistant tumors were harvested and total mRNA analyzed for Sox2 and PSA. These data show increased Sox2 within castration-resistant LAPC-4 and CWR-R1 tumors (*p<0.05; data represents quantitation from multiple tumor specimens).

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) To test whether Sox2 expression is associated with resistance to AR pathway inhibition, we developed a series of prostate cancer cell lines that were resistant to MDV3100. After 30 days of continuous treatment, MDV3100-resistant lines expressed significantly higher Sox2 as measured using qPCR (*p<0.05). AR-mediated PSA expression was also significantly reduced in these lines. B ) Comparison between the castration-sensitive LNCaP and isogenic castration-resistant C4-2 cell lines documents a significant increase in Sox2 expression in castration-resistant C4-2 cells (*p<0.05). C ) Increased Sox2 expression in castration-resistant xenografts of LAPC-4 and CWR-R1 tumors. Tumors were allowed to establish and then host mice were castrated; 30 days later castration-resistant tumors were harvested and total mRNA analyzed for Sox2 and PSA. These data show increased Sox2 within castration-resistant LAPC-4 and CWR-R1 tumors (*p<0.05; data represents quantitation from multiple tumor specimens).

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Expressing, Inhibition, Comparison, Quantitation Assay

A ) Ectopic expression of Sox2 in castrate-sensitive LAPC-4 (LV-Sox2, n = 10 mice) cells is sufficient to promote tumor take in castrated male nude hosts compared to control mice (LV-Control, n = 10) (p = 0.019). Inset: western blot documenting ectopic lentiviral Sox2 expression in LAPC-4 cells. GAPDH was used as a loading control. NCCIT cells are used as a Sox2-positive control. B ) Castrated LAPC-4-Sox2 versus LAPC-4-Control tumors do not have differences in serum PSA density. These data support that Sox2 expression does not confer a less-differentiated tumor phenotype. PSA density is the ng/mL total PSA per gram tumor. C ) Expression of Sox2 in LAPC-4 does not significantly increase the percentage of rare CD133-positive cells, which are thought to be putative cancer stem/initiating cells.

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) Ectopic expression of Sox2 in castrate-sensitive LAPC-4 (LV-Sox2, n = 10 mice) cells is sufficient to promote tumor take in castrated male nude hosts compared to control mice (LV-Control, n = 10) (p = 0.019). Inset: western blot documenting ectopic lentiviral Sox2 expression in LAPC-4 cells. GAPDH was used as a loading control. NCCIT cells are used as a Sox2-positive control. B ) Castrated LAPC-4-Sox2 versus LAPC-4-Control tumors do not have differences in serum PSA density. These data support that Sox2 expression does not confer a less-differentiated tumor phenotype. PSA density is the ng/mL total PSA per gram tumor. C ) Expression of Sox2 in LAPC-4 does not significantly increase the percentage of rare CD133-positive cells, which are thought to be putative cancer stem/initiating cells.

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Expressing, Control, Western Blot, Positive Control

A ) Depletion of Sox2 protein expression using four different lentiviral shRNA constructs. To test the impact of inhibiting Sox2 expression in castration-resistant CWR-R1 cells, we used a series of shRNAs that targeted different regions of the Sox2 mRNA transcript. These data show that each shRNA sequence results in decreased Sox2 protein expression after 72 hours, and combinations of three were used to obtain levels below detection while controlling for potential off-target (i.e. non-Sox2 specific) effects. A non-silencing shRNA (NSC) control was used in comparison. GAPDH was used as a loading control. B ) Decreased expression of Sox2 results in significant growth inhibition. Cell growth was measured using MTT reduction after five days in complete media. In all four instances of Sox2 knockdown, cell growth was significantly diminished compared to the non-silencing control.

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) Depletion of Sox2 protein expression using four different lentiviral shRNA constructs. To test the impact of inhibiting Sox2 expression in castration-resistant CWR-R1 cells, we used a series of shRNAs that targeted different regions of the Sox2 mRNA transcript. These data show that each shRNA sequence results in decreased Sox2 protein expression after 72 hours, and combinations of three were used to obtain levels below detection while controlling for potential off-target (i.e. non-Sox2 specific) effects. A non-silencing shRNA (NSC) control was used in comparison. GAPDH was used as a loading control. B ) Decreased expression of Sox2 results in significant growth inhibition. Cell growth was measured using MTT reduction after five days in complete media. In all four instances of Sox2 knockdown, cell growth was significantly diminished compared to the non-silencing control.

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Expressing, shRNA, Construct, Sequencing, Control, Comparison, Inhibition, Knockdown

A ) The expression of 83 embryonic stem cell-associated genes was analyzed via quantitative real-time PCR analyses to identify Sox2-associated gene changes in LAPC-4-Sox2 cells (compared to LAPC-4-Control cells). Open bars represent genes that are previously identified Sox2-target genes in hESCs (Boyer et al. 2005). No changes in any of these 18 genes were associated with Sox2 expression in LAPC-4 cells. Rather, significant changes in expression were detected for FGF5, Kit, NR5A2, PDX1, and RUNX2 (black bars). B ) Elevated expression of FGF5 in castration-resistant LAPC-4 and CWR-R1 tumors (*p<0.05; same mRNA as described in ).

Journal: PLoS ONE

Article Title: Sox2 Is an Androgen Receptor-Repressed Gene That Promotes Castration-Resistant Prostate Cancer

doi: 10.1371/journal.pone.0053701

Figure Lengend Snippet: A ) The expression of 83 embryonic stem cell-associated genes was analyzed via quantitative real-time PCR analyses to identify Sox2-associated gene changes in LAPC-4-Sox2 cells (compared to LAPC-4-Control cells). Open bars represent genes that are previously identified Sox2-target genes in hESCs (Boyer et al. 2005). No changes in any of these 18 genes were associated with Sox2 expression in LAPC-4 cells. Rather, significant changes in expression were detected for FGF5, Kit, NR5A2, PDX1, and RUNX2 (black bars). B ) Elevated expression of FGF5 in castration-resistant LAPC-4 and CWR-R1 tumors (*p<0.05; same mRNA as described in ).

Article Snippet: Knockdown of Sox2 expression using shRNA expression was achieved using the anti-human Sox2 shRNA gene set, which consists of 4 different lentiviral targeting sequences against Sox2 and a non-silencing control [HSH017628-HIVH1(Sox2) and CSHCTR001-HIVH1 (control) vectors containing a GFP and puromycin-resistance components; Genecopoeia].

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control